Buffo is an Italian-speaking water buffalo who invents gadgets, yet he can also be clumsy. He also assists Oso in training exercises. He appears in For Sleepy Eyes Only.
- Buffo first appeared in "Dr. Juice" in TV, but in The Oso Comics, he first appeared in Oso in Clipped Bored .
- Buffo he is voiced by Brad Garrett, who also voiced Dim in A Bug's Life, Bloat in Finding Nemo, Fatso in Casper, Gusteau from Ratatouille, Riff Raff from Underdog Luca in Garfield: The Movie, and The Giant from Hoodwinked Too! Hood Vs Evil. Easter Island Head from Night at the Museum, Night at the Museum: Battle of the Smithsonian.